Born in 1990 in Austria, Mario Rom received his first trumpet lessons from his father, Bernd Rom. From 2004 to 2013, he studied at the Anton Bruckner Private University under Josef Eidenberger and took additional lessons from, among others, Paul Pawluk, Hedi Milek, Laurie Frink, Paul Mayes, Thomas Gansch, Ralph Alessi, and Jörg Engels.

In 2011, he founded the band Mario Rom's Interzone together with Lukas Kranzelbinder and Herbert Pirker, which has released four albums to date. The band has been invited to numerous international jazz festivals, including the Montreal Jazz Festival, and has performed concerts worldwide in the USA, Canada, South Africa, Argentina, India, China, Qatar, Tunisia, and Morocco. In 2022, the band collaborated with the legendary musician Danyèl Waro from La Reunion as part of Glatt und Verkehrt festival. The recording of this concert was released in 2024.

Rom is also a member of the band Shake Stew, which won the German Jazz Prize in 2021 for "best international band" and the Amadeus Award in 2023. Additionally, he is part of the bands Memplex and Wolfgang Muthspiel's Chamber Trio and regularly plays with Dhafer Youssef.

In 2018, he participated in the SWR New Jazz Meeting and has collaborated with numerous other artists, including Wolfgang Reisinger, Colin Vallon, Shabaka Hutchings, David Murray, Kirk Lightsey, Vienna Art Orchestra, Christoph Cech, Mathias Rüegg, Golnar Shayhar, Lia Pale, Christian Muthspiel, Dave Liebman, Jazzwerkstatt Wien, Christian Reiner and Vlado Dzihan.

He was featured in Erwin Wagenhofer's documentary film "But Beautiful" (2019) and, along with Lucia Pulido and Kenny Werner, contributed to the film's soundtrack.

Since November 2019, Mario Rom is a professor of jazz trumpet at the Institute for Jazz and Improvised Music at the Anton Bruckner Private University in Linz.